To further examine the function of PCK1 in steatosis in vitro, we overexpressed (PCK1-OE) using the AdEasy adenoviral vector system and knocked out PCK1 (PCK1-KO) using the CRISPR-Cas9 system in human hepatocytes (Supplementary Fig. 5c, d). The gene discussed is PCK1; the disease is steatosis.