ALT, AST, IL-6, TNF-a, TC, TG, and FFAs levels significantly increased in the serum and liver tissues of cPtenf/fPck1f/f mice, suggesting more serious liver injury and lipid metabolism disorder in cPtenf/fPck1f/f mice (Supplementary Fig. 3d, e). This evidence concerns the gene TNF and Disorder of lipid metabolism.