To further evaluate the involvement of RhoA in HSC activation, we isolated primary hepatocytes from HFCD-HF/G-fed WT or L-KO mice; treated the cells with MK2206, Rhosin, or dimethyl sulfoxide vehicle; and then co-cultured the cells with primary HSCs from chow-fed WT mice. The gene discussed is RHOA; the disease is hydrops fetalis.