PCK1 and obesity due to melanocortin 4 receptor deficiency: To determine whether PCK1 is involved in MAFLD, we first examined hepatic gene expression in a published transcriptome dataset [Gene Expression Omnibus (GEO): GSE126848] containing samples from 14 healthy participants, 12 patients with obesity, 15 patients with NAFLD, and 16 patients with NASH16.