After 10 weeks of chow diet or HFCD-HF/G feeding, WT and L-KO mice were injected through the tail vein with AAV serotype 8 (AAV8) vector expressing Pck1 under the control of a liver-specific promoter (thyroxine-binding globulin, TBG), AAV8-TBG-Pck1 or AAV8-TBG-control (Fig. 3i). Here, PCK1 is linked to hydrops fetalis.