The results showed that, without additional infection, loss of Brd9 led to slight inhibition on the basal expression of proinflammatory cytokines, such as TNF-α, iNOS, CD86, IL-1b with no apparent effect on RANKL in the femur bone tissue as shown with immunofluorescence staining, as well as the quantification analysis using qPCR (Supplementary Fig. 7). Here, TNF is linked to infection.