TSPYL2 and cancer: Indeed, some male cancer cell lines showed a reduction of TSPYL2 after etoposide (e.g. A549, DU145, Jurkat and HT1080), while in others (e.g. MG-63 and HCT116) TSPYL2 decreased at 24 h after damage and then accumulated at 48 h (Fig. 1F).