ERN1 and melanoma: Further, we treated SIRT7-overexpressed WM35 melanoma cell with IRE1α-specific inhibitor STF083010 under ER stress and found that STF083010 could reverse the SIRT7 overexpression-mediated ERK activation and XBP1s up-regulation (Fig. 3g), which suggested that the activation of the ERK by SIRT7 was in an IRE1α-dependent manner.