NFKB1 and melanoma: Pharmacological inhibition of NF-κB p65 by pyrrolidinedithiocarbamate ammonium (APDC) led to reduced phosphorylation of p65, paralleled with significant down-regulation of SIRT7 mRNA and protein levels (Fig. S10c, d), indicating that NF-κB p65 was responsible for SIRT7 up-regulation in melanoma under normal condition.