B16F10 melanoma cells were subcutaneously implanted into C57BL/6 mice with or without the stable knockdown of SIRT7, and the mice then received the intraperitoneal injection with HA15 to trigger ER stress that mimics the stressful TME (Supplementary Fig. S7a).46 Flow cytometry analysis was employed to analyze the immune cells in implanted tumors, including CD8+T cells, macrophages, MDSC DCs, and Tregs. Here, CD8A is linked to melanoma.