The top 10 pathways obtained by KEGG analysis were: protein digestion and absorption, proteoglycans in cancer, focal adhesion, human papillomavirus infection, PI3K-Akt signaling pathway, ECM-receptor interaction, cell adhesion molecules, axon guidance, cAMP signaling pathway, and vascular smooth muscle contraction (Fig. 4B, Additional file 10: Table S14). The gene discussed is AKT1; the disease is cancer.