MLKL and colorectal carcinoma: Additionally, the expression of necroptosis markers such as receptor-interacting protein-1 (RIP-1), RIP-3, and phosphorylated Mixed lineage kinase domain-like protein (MLKL) at Ser358 were also increased (Fig. 2C), suggesting that SNG can trigger both features of apoptosis and necroptosis in human CRC cells.