To validate our findings in a different cohort, we extracted data of 53 patients with NPC from the GEO database and analyzed the correlation of HAVCR2 (TIM3), TIGIT, 2B4, PDCD, KLRG1, CTLA4, CD244, BTLA, CD160, and LAG3 on the mRNA level. The gene discussed is CTLA4; the disease is nasopharyngeal carcinoma.