It is also worth noting that the parental B16 cell line does not express TLR9 (S1 Fig) and that upon inoculation of mice, B14.3 HER2 tumor cells maintain a moderate degree of human HER2 expression (Fig 6B) but show a decrease in HER2 expression compared to cultured cells (S1 Fig). Here, TLR9 is linked to neoplasm.