POLE and non-small cell lung carcinoma: identified 48 genes whose mutations were primarily associated with increased TMB in human cancers, among which there were many DNA mismatch repair pathway genes and DNA polymerase genes, including MSH2, MSH6, MLH1, PMS2, and POLE. In another exploration of the association between somatic genetic alterations and TMB in 513 non-small-cell lung cancers (NSCLC) (16), Zhu et al.