Based on the mutation status of the three genes and gender status, we defined the upgrade TMB prognostic score (UTMBPS) as: UTMBPS = - (a × mutated (ROS1) + b × mutated (SPEN) + c × mutated (PTPRT) + d × gender), where a = -0.7131, b = -0.7369, c = -0.4941, and d = 0.1890, which were the coefficients of the four variables in the Cox proportional hazards model: mutated(X) = 1 if the gene X is mutated in the tumor sample, otherwise mutated(X) = 0; gender(X) = 1 if the patient is female, otherwise gender(X) = 0. Here, SPEN is linked to neoplasm.