We observed NLRP6-/-NOD and NLRP6+/+ littermates for spontaneous diabetes, and observed significantly delayed and reduced T1D development in female NLRP6-/- mice with reduced immune infiltration of the pancreas (Figure 1A and Supplementary Figure 1A) compared to NLRP6+/+ mice. This evidence concerns the gene NLRP6 and type 1 diabetes mellitus.