In the process of NASH fibrosis, some types of monocytes in the MMD system transformed into new monocytes, macrophages and DC cells, and Fmnl1 and Myh9 levels in the MMD system were also significantly increased at the end of the trajectory path, which was associated with the deposition of liver collagen in NASH mice and patients with fibrosis. The gene discussed is MYH9; the disease is metabolic dysfunction-associated steatohepatitis.