PRF1 and lung adenocarcinoma: When we compared the synapses formed between NK cells from healthy donors and A549 (a lung adenocarcinoma line) fixed after 5 min of interaction (Figure 4A), the perforin granules had gathered around the MTOC to a similar extent in all experimental treatments (Figure 4B), but the MTOC polarized to the synapse more frequently in the case of TriKE treatment, compared to NK cells treated with IL-15 or left untreated (Figure 4C).