However, unexpectedly, we discovered that the majority of HCC‐specific genes in HepG2 (HCC) cells have concomitant increase of CTCF binding comparing to THLE2 cells, whereas the HCC‐suppressed genes showed higher CTCF binding and looping in THLE2 cells than in HepG2 cells. This evidence concerns the gene CTCF and hepatocellular carcinoma.