We found that the HepG2‐ and THLE2‐specific CTCF binding peak sites (≈500 bp) and CTCF binding motifs (219 bp) proximal to the TSS of HCC‐specific genes in HepG2 and HCC‐suppressed genes in THLE2 are both hypomethylated, respectively (Figure 5A,B), which is in line with the overall CTCF binding peaks in HepG2 and THLE2 cells, respectively (Figure S5D,E, Supporting Information). The gene discussed is CTCF; the disease is hepatocellular carcinoma.