A number of known HCC marker genes (AFP, GPC3, CCL15, HULC, Table S2, Supporting Information) are indeed found highly expressed in HepG2 cells, while the expression of several normal liver marker genes (SAA1, SAA2, NNMT, CXCL2, Table S2, Supporting Information) are significantly lower in HepG2 cells and higher in THLE2 cells. The gene discussed is GPC3; the disease is hepatocellular carcinoma.