Furthermore, ANOVA tests on the tumor‐enriched clusters revealed that CD8‐c09‐Isg15, CD8‐c10‐Cd244, CD8‐c11‐Ccr7, CD8‐c12‐Gzmc, CD4‐c09‐Gzmb, Macro‐c03‐Ifit3, and NK‐c03‐Ccl5 were impacted by combined effects of liver tumors and anti‐PD‐1 treatment (Figure 5G). This evidence concerns the gene PDCD1 and neoplasm.