Nonetheless, we performed MR-Egger regression, MR.RAPS and CAUSE analyses, which were more robust to invalid SNPs and considered the correlated and uncorrelated pleiotropy effects; fourth, the difference in the sample size of GWAS-VEGF and GWAS-malignant brain tumor may lead to unstable statistical results, and the identified SNPs may exhibit potential weak instrument bias, but this is less likely because the F statistics for each SNP used was significantly higher than ten. The gene discussed is VEGFA; the disease is brain cancer.