The main MR methods indicated that VEGF was not associated with malignant brain tumors (IVW: OR = 1.277, 95% CI, 0.812~2.009, P = 0.289) without heterogeneity (Q_pval = 0.572, I2 = 0.000) and horizontal pleiotropy (intercept = 0.054, P = 0.654). The gene discussed is VEGFA; the disease is brain cancer.