To investigate the probable involvement of ADAM8 in the tumor microenvironment, pancreatic ductal adenocarcinoma (PDAC) cancer cells, such as Panc89 and AsPC1 cells, have been an engineered CRISPR/Cas9 knockout of ADAM8 and their liberation of extracellular vesicles has been scrutinized. Here, ADAM8 is linked to pancreatic ductal adenocarcinoma.