In the current study, we explored the alteration of CD4 + CD25 + CD127 low Treg, nTreg (CD4 + CD25 + CD127 low CD45RA+) and mTreg (CD4 + CD25 + CD127 low CD45RO+) in AIS and investigated their role in stroke outcome prediction. Here, CD4 is linked to androgen insensitivity syndrome.