In light of this, we performed immunofluorescence staining for Iba1, a microglia-specific marker, and found that the number of activated microglia, which possessed a larger volume of soma as well as shorter and bolder processes, were markedly increased in the Dlg1 f/f mice subjected to CRS (Figures 3A–C). Here, AIF1 is linked to congenital rubella syndrome.