The results showed that TLR4, NF-κB, and Myd88 proteins were up-regulated in NASH rat primary hepatocytes, and the QTHX down-regulated the TLR4, NF-κB, and Myd88 proteins (Figure 4(A)), and the qPCR results were consistent with the protein results (Figure 4(B)), indicating that QTHX can inhibit the TLR4/NF-κB cell signalling pathway. The gene discussed is NFKB1; the disease is metabolic dysfunction-associated steatohepatitis.