The TIMER2.0 server was applied, and as shown in supplementary figure 2, CALR expression was positively related to tumor-infiltrating immune cells including M1 macrophages (R = 0.160, P ˂ 0.05), DCs (R = 0.178, P ˂ 0.05), CD8+ (R = 0.164, P ˂ 0.05), and CD4+ (R = 0.243, P ˂ 0.01) memory T cells, whereas there was a negative correlation between CALR expression and M2 macrophages in tumor microenvironment (R = −0.15, P ˂ 0.05). The gene discussed is CD4; the disease is neoplasm.