We have demonstrated that the culture medium of B cells treated with 100 μM Hcy for 72 h, which contains high level anti-β2GPI antibody, and its purified IgG both induced a shift in macrophage phenotypes towards M1 polarity and evoked vascular inflammation.18 GECs were treated with con-B CM or Hcy-B CM (culture medium of B cells treated with or without 100 μM Hcy for 72 h) and with or without Ang II for 24 h to simulate the in vivo environment of renal hypertension and HHcy (Supplementary Fig. 3a). This evidence concerns the gene AGT and renal hypertension.