Further, stage II obesity participants demonstrated significantly higher levels of plasmatic leptin, serum glucose, and lower levels of plasmatic ghrelin with respect to stage I obesity (p < 0.001, p = 0.001, and p < 0.001, respectively) and significantly higher levels of plasmatic leptin, insulin, and serum glucose and lower levels of plasmatic ghrelin with respect to OW (p < 0.001, p = 0.002, p < 0.001, and p < 0.001, respectively) and LS (p < 0.001, p < 0.001, p < 0.00,1 and p < 0.001, respectively). This evidence concerns the gene INS and obesity due to melanocortin 4 receptor deficiency.