To further validate their results in the context of cancer, Rabinovich et al. [10], investigated osteosarcoma cell lines either proficient (U2OS) or deficient (MNNG/HOS) in ASS1, showing a similar increase in proliferation with reduced ASS1 expression, associated with higher uracil levels and pyrimidine synthesis. The gene discussed is ASS1; the disease is osteosarcoma.