VCAM1 and metabolic dysfunction-associated steatohepatitis: Therefore, we first fed wild-type mice with a control diet (ND) or a methionine-low, choline-deficient high-fat diet (HCD) for 6 weeks, as described in the Materials and Methods, to induce NASH, and we assessed the expression of α4 integrin, the receptor of VCAM-1, on different leukocyte subpopulations, utilizing flow cytometry.