NFKB1 and colonic neoplasm: Similarly, in human colon cancer, HCT116, HT29, and SW620 cell lines, a significant decrease in factors related to NF-κB signaling such as nuclear p65, p50, p-IKKα/β, and p-IκBα was observed when the cells were treated with cyanidin chloride at a concentration of 50 μM [135].