Our functional studies in NSC-34 motor neuron-like cells by transfection with miR-146a inhibitor demonstrated a relationship between miR-146a-5p and Nfl gene expression, as observed for miR-146a-3p, suggesting that Nfl changes may be an effect of miR-146a-5p dysregulation in ALS mice. The gene discussed is NEFL; the disease is amyotrophic lateral sclerosis.