Wang et al. developed efficient and reproducible agarose hydrogel microwells to produce uniform-sized multi-cellular tumour spheroids, which offer better mimicry of traditional solid tumours and allow the evaluation of some anti-cancer drug candidates’ effects, starting from cells of HCC-patients with abnormally high expression of fibroblast growth factor receptor 4 (FGFR4) [31]. This evidence concerns the gene FGFR4 and hepatocellular carcinoma.