Our study demonstrates that TIM-3, TIGIT, and CTLA-4, overexpressed in the patients with B-ALL, correlate not only with transcriptional factors and cytokines expressed by Tregs, but also with CD8α and β chain+ T cells, suggesting that these markers are expressed not only by CD4+ Treg, but also by CD8+ T cells. This evidence concerns the gene TIGIT and precursor B-cell acute lymphoblastic leukemia.