Additionally, it was found that about 57% of the O2.− concentration measured in protoplasts inoculated with Pst DC3000 (avrRPM1) was caused by NADPH oxidase activity since the use of DPI significantly reduced the formazan content in Arabidopsis protoplasts (Supplemental Table S3), revealing that there are different sources of ROS production with specific allocation, temporality, and intensity in order to confront the bacterial infection. This evidence concerns the gene FMO5 and bacterial infectious disease.