To elucidate the mechanisms of GCK knockdown on hepatic lipid metabolism, the expression of proteins responsible for de novo lipogenesis (FASN and ACC), lipolysis (ATGL), β-oxidation of fatty acids (PPARα and CPT-1) and the Kennedy pathway for PC synthesis (CHPT-1) in the human HepG2 cells and mouse AML-12 cells was investigated using Western blot. The gene discussed is FASN; the disease is acute myeloid leukemia.