Finally, the expression of the revealed hub genes related to acute colitis (C3, Tyrobp, Mmp3, Mmp9, Timp1) and CAC (Timp1, Adam8, Mmp7, Mmp13) was validated by qRT-PCR in the colon tissue of mice with acute colitis and colitis-driven adenomas (Figure 3D). This evidence concerns the gene MMP3 and colitis.