Given that previous results showed GBE1 negatively regulated FBP1 expression in U251 cells (Figure 4E,F), to further verify the role of FBP1 in gliomas, we constructed U251 cells with FBP1 knockdown (sh-FBP1) and FBP1 overexpression (oe-FBP1). The gene discussed is FBP1; the disease is glioma.