To further investigate the role of GBE1 in gliomas, stable GBE1 knockdown and negative control U87, ln229, and U251 cells were constructed by sh-GBE1-1, sh-GBE1-2, and sh-NC lentiviruses, and the knockdown efficiency was verified by qPCR and WB (Figure 2A–C). Here, GBE1 is linked to central nervous system cancer.