ASCL1 and small cell lung carcinoma: We visualized the SCLC scores in the E–M space obtained from nnPCA (Figure 4A) and found that while there was a distinct, small population corresponding to an M-like state as previously reported [9], most tumor cells were located in a continuous region with relatively high A2 and A scores (Figure 4A), consistent with their positivity for ASCL1 as previously shown.