GLI1 and colorectal carcinoma: In BRAFV600E-driven CRC, both pathways were upregulated after 5-FU treatment, but importantly GANT61 downregulated not only its specific target GLI1 but also NOTCH (Figure 2A), suggesting an upstream role of HH-GLI over the NOTCH pathway (Figure 6), thus explaining the positive correlation between these two signaling pathways (Figure 2C).