To assess whether the immune cells that infiltrated the tumor microenvironment in the drug-treated isografts have the capability to produce cytokines, the single cell suspensions from the control and NSC243928 treated tumor isografts were subjected to an ex vivo treatment with PMA and ionomycin for 4 h before flow cytometry analysis for TNFα, IFNγ, and CD107(LAMP1) on the CD4 and CD8 positive (+) T cells. The gene discussed is CD4; the disease is neoplasm.