Two VUS were recurrently detected in three patients (RUNX1 NM_001754.4:c.1270T > C) and two patients (ZRSR2 NM_005089.3:c.283G > A) that need further investigation to elucidate their roles in the leukaemogenesis of AML. This evidence concerns the gene ZRSR2 and acute myeloid leukemia.