In the case of HCC, the expression level of BUB1, CCNA2, CCNB2, KIF2C, MELK, NCAPG, NCAPH, NUF2, PBK, and TOP2A was higher due to their lower promoter methylation level against normal samples while ASPM, AURKA and BUB1B were lowly expressed (see Supplementary Figure S4b). This evidence concerns the gene ASPM and hepatocellular carcinoma.