To investigate this further, mRNA expression analyses of several possible linked molecules including (1) oncogenic signaling related factors (KRAS, SOX2, MITF, BRAF, FOS, and STAT3), (2) PCG1α, and (3) ECMs (COL4, COL6, FN, and αSMA) and tight-junction (TJ) related molecule (ZO-1) were carried out among 2D and 3D cultured five MM cell lines (Table 4 and Figure S5). Here, TJP1 is linked to Miyoshi myopathy.