In the univariate Cox analysis for OS in both the FU-iCCA cohort and the ZSH cohort, vascular invasion (both P < 0.001), LN metastasis (both P < 0.001), large tumor size (both P < 0.05), elevated serum CA199 and CEA levels (both P < 0.001), advanced AJCC 8th stage (P < 0.001), and high CD73 expression (both P < 0.001) were found to have a significant correlation with unfavorable OS. This evidence concerns the gene NT5E and neoplasm.