Prior to in vitro studies, endogenous expression of CDKL3 in normal (WPMY-1) as well as tumor cells including DU 145, PC-3, and LNcap was detected by qPCR, which showed that CDKL3 expression was significantly lower in normal cells than in tumor cells (Fig. 1B), which was also consistent with clinical tissue-related results. The gene discussed is CDKL3; the disease is neoplasm.