To further explore the tumor suppressor role of TINCR in human SCCs, we evaluated the impact of mimicking P53-induced TINCR upregulation by lentivirally expressing the TINCR protein-coding sequence in TP53 mutant CAL-27 (TP53 homozygous c.578 A > T) and FaDu (TP53 c.376-1 G > A, c.743 G > T) head and neck squamous carcinoma (HNSCC) cell lines, which lack the expression of endogenous TINCR (Supplementary Fig. 3d, e). Here, TINCR is linked to head and neck squamous cell carcinoma.