To make sure the key domain of PTPN14 in promoting gastric cancer progression, we subcloned three PTPN14 mutant plasmids: PTPN14-ΔFERM (deletion of the FERM domain, 17–310 amino acids), PTPN14-YA/YA mutant (the PPxY motif mutant, Y570A/Y752A), and PTPN14-ΔPTP (deletion the PTP domain, 934–1187 amino acids) (Fig. 4A). This evidence concerns the gene PTPN14 and gastric cancer.