To determine the contribution of endogenous IFNε expression in the FRT, relative to other (conventional) type-I IFNs to prevent ZIKV infection, we compared the outcomes of intravaginal (iVag) infection [20] of wildtype (WT), IFNε-/- or IFNAR1-/- mice with 5 X 105 FFU of ZIKV Puerto Rican stain PRVABC59 (Fig 1A). This evidence concerns the gene IFNAR1 and Zika virus infectious disease.