In the results of the three algorithms for protein function prediction (Fig. 2, Supplementary Table 1, Supplemental digital content 2, http://links.lww.com/EJCP/A381 and Supplementary Table 2, Supplemental digital content 3, http://links.lww.com/EJCP/A382), the predicted impact of VUS on gene expression in patients with pancreatic cancer included predicted possibly damaging and deleterious variants in MLH1, MSH2 and MSH6. Here, MSH2 is linked to pancreatic neoplasm.