In the results of the three algorithms for protein function prediction (Fig. 2, Supplementary Table 1, Supplemental digital content 2, http://links.lww.com/EJCP/A381 and Supplementary Table 2, Supplemental digital content 3, http://links.lww.com/EJCP/A382), the predicted impact of VUS on gene expression in patients with pancreatic cancer included predicted possibly damaging and deleterious variants in MLH1, MSH2 and MSH6. The gene discussed is MSH2; the disease is familial pancreatic carcinoma.