Figure 4A shows volcano plots of the DEGs between each group. Under the SCD, cathepsin S (Ctss), endothelial cell adhesion molecule (Esam), and minichromosome maintenance complex component 6 (Mcm6) were the top three upregulated genes, and aspartate beta-hydroxylase (Asph), ribosomal protein S (Rps21), and BRCA-associated RING domain 1 (Bard1) were the top three downregulated genes after SD. The mcm6, C-C motif chemokine ligand 4 (CCl4), and interleukin 1 receptor kinase 3 (Irak3) genes were upregulated in the EC+HFD group compared to those in the EC+SCD group (Figure 4A). Here, CTSS is linked to Schnyder corneal dystrophy.