We intranasally infected mice at ZT3 and ZT12, time of low and high expression of Ido1, and collected tissues at 1, 3, and 7 days post-infection (sacrifice of mice infected at ZT3 was performed at ZT3; sacrifice of mice infected at ZT12 was performed at ZT12) (Fig. 3A). This evidence concerns the gene IDO1 and infection.