qPCR and Multiplex immunoassay performed in lung homogenates confirmed that, in line with cytospin analysis, infection at ZT12 was associated with lower levels of expression of pro-inflammatory cytokines TNFα, IL-6, and IL-1β (Fig. 3F) and the chemokine CXCL1 (Figures S4B and S5). This evidence concerns the gene CXCL1 and infection.