Once the tumor volumes had reached approximately 80–100 mm3 size, PBS, non-transduced NK92 cells, NC-NKG2D-CAR-NK92 and SH-NKG2D-CAR-NK92 cells (1 × 107 cells/mouse) were injected i.v. into the tumor-bearing mice and each mouse was intraperitoneally injected with 20000 IU of IL-2. Here, KLRK1 is linked to neoplasm.