CD274 and colorectal carcinoma: In addition, the implication of a few results remains un-explained such as the negative CD68 markers and NK markers in tumor tissues of samples with PD-L1 deletions; How would PD-L1 deletion affect macrophage or NK cell infiltration and alter the tumor microenvironment is unknown; Finally, the predictive value of PD-L1 genetic alterations in anti-PD-1 therapy efficacy warrants further study in CRC patients with anti-PD-1 therapy.