RPA and RAD52 co-localized at the telomeres of ALT-positive cancer cells marked by telomere repeat binding factor 2 (TRF2), and their association with telomeres was further enhanced by replication stress or when ALT activity was increased by FANCM depletion, as has been demonstrated previously43,44 (Extended Data Fig. 8a–j). This evidence concerns the gene TERF2 and cancer.