RSPO1 and steatosis: Histological analyses of the liver showed that the area of steatosis, the degree of lobular inflammation and hepatocellular ballooning, and the area of fibrosis were all decreased significantly in R-Spo1-treated CDAHFD mice (CDAHFD + R-Spo1 group) compared to CDAHFD-fed mice treated with PBS (CDAHFD + PBS group) (Fig. 5a,b).